human CYP8B1 shRNA silencing AAV

Name
AAV-h-CYP8B1-shRNA

Cat No
shAAV-206532

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CYP8B1.

shAAV-206532

AAV-h-CYP8B1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CYP8B1 (cytochrome P450 family 8 subfamily B member 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CP8B; CYP12

Description (Vector)

CYP8B1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. CYP8B1 is unique among the cytochrome P450 genes in that it is intronless.

Gene ID

1582

Gene Name (Vector)

cytochrome P450 family 8 subfamily B member 1

Gene Symbol

CYP8B1

HGNC ID

HGNC:2653

ORF Size (aa)

1506

ORF Size (bp)

1506 bp

RefSeq ID

NM_004391

RefSeq Synonyms

NM_004391, BC067444, BC067442, BC067441, BC067434,

Species

human

UniGene ID

Hs.447793

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.