human CYP7A1 shRNA silencing AAV

Name
AAV-h-CYP7A1-shRNA

Cat No
shAAV-206529

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CYP7A1.

shAAV-206529

AAV-h-CYP7A1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CYP7A1 (cytochrome P450 family 7 subfamily A member 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CP7A; CYP7; CYPVII

Description (Vector)

CYP7A1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis.

Gene ID

1581

Gene Name (Vector)

cytochrome P450 family 7 subfamily A member 1

Gene Symbol

CYP7A1

HGNC ID

HGNC:2651

ORF Size (aa)

1515

ORF Size (bp)

1515 bp

RefSeq ID

NM_000780

RefSeq Synonyms

NM_000780, BC112184, BC101777,

Species

human

UniGene ID

Hs.1644

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.