human CTSL shRNA silencing AAV

Name 
AAV-h-CTSL-shRNA
Cat No 
shAAV-206277
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human CTSL.

shAAV-206277
AAV-h-CTSL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CTSL (cathepsin L). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CATL; CTSL1; MEP
Description (Vector)
CTSL1 is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene.
Gene ID
1514
Gene Name (Vector)
cathepsin L
Gene Symbol
CTSL
HGNC ID
HGNC:2537
ORF Size (aa)
1002
ORF Size (bp)
1002 bp
RefSeq ID
NM_001912
RefSeq Synonyms
NM_145918, NM_001912, NM_001257973, NM_001257972, NM_001257971, BC142983, BC012612,
Species
human
UniGene ID
Hs.716407

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.