human CTCF shRNA silencing AAV

Name 
AAV-h-CTCF-shRNA
Cat No 
shAAV-206223
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human CTCF.

shAAV-206223
AAV-h-CTCF-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CTCF (CCCTC-binding factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCFL paralog; MRD21; transcriptional repressor CTCF
Description (eCommerce)
CTCF is a versatile 11-Zn-finger transcription factor that may function as a repressor of transcription, activator transcription, or regulator of genomic imprinting. CTCF is a candidate tumor suppressor associated with invasive breast cancer, prostate cancer, and Wilm’s tumor.
Description (Vector)
CTCF is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene ID
10664
Gene Name (eCommerce)
CCCTC-binding factor
Gene Name (Vector)
CCCTC-binding factor
Gene Symbol
CTCF
HGNC ID
HGNC:13723
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2184
ORF Size (bp)
2184 bp
Protein Name (eCommerce)
Transcriptional repressor CTCF
RefSeq ID
NM_006565
RefSeq Synonyms
NM_006565, NM_001191022, BC014267,
Research Areas
Cancer,Cell Cycle,Chromatin Remodeling,Epigenetic Regulation of Gene Expression,Gene Expression,Histone Modification,Neurobiology,Signal Transduction,TGF-beta Signaling,Transcription Factor/Regulator,Tumor Suppressors/Oncoproteins,Zinc-finger
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,genetics,transcription_translation,immunology,neurobiology,signal_transduction
Species
human
Target Sentence
CTCF is a versatile 11-Zn-finger transcription factor that may function as a repressor of transcription, activator transcription, or regulator of genomic imprinting. CTCF is a candidate tumor suppressor associated with invasive breast cancer, prostate cancer, and Wilm’s tumor.
UniGene ID
Hs.368367
UniProt ID (eCommerce)
P49711

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.