human CLDN18 shRNA silencing AAV

Name
AAV-h-CLDN18-shRNA

Cat No
shAAV-205374

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CLDN18.

shAAV-205374

AAV-h-CLDN18-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CLDN18 (claudin 18). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

SFTA5; SFTPJ

Description (Vector)

CLDN18 encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. CLDN18 is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Gene ID

51208

Gene Name (Vector)

claudin 18

Gene Symbol

CLDN18

HGNC ID

HGNC:2039

ORF Size (aa)

786

ORF Size (bp)

786 bp

RefSeq ID

NM_016369

RefSeq Synonyms

NM_016369, NM_001002026, BC146668, BC142708,

Species

human

UniGene ID

Hs.655324

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.