human CIITA shRNA silencing AAV

Name
AAV-h-CIITA-shRNA

Cat No
shAAV-205283

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human CIITA.

shAAV-205283

AAV-h-CIITA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CIITA (class II major histocompatibility complex transactivator). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

C2TA; CIITAIV; MHC2TA; NLRA

Description (Vector)

CIITA encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the 'master control factor' for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction.

Gene ID

4261

Gene Name (Vector)

class II major histocompatibility complex transactivator

Gene Symbol

CIITA

HGNC ID

HGNC:7067

ORF Size (aa)

3393

ORF Size (bp)

3393 bp

RefSeq ID

NM_000246

RefSeq Synonyms

NM_001286403, NM_001286402, NM_000246, BC172348,

Species

human

UniGene ID

Hs.701991

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.