human CERKL shRNA silencing AAV

Name
AAV-h-CERKL-shRNA

Cat No
shAAV-205017

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CERKL.

shAAV-205017

AAV-h-CERKL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CERKL (ceramide kinase like). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

RP26

Description (Vector)

CERKL was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. CERKL encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.

Gene ID

375298

Gene Name (Vector)

ceramide kinase like

Gene Symbol

CERKL

HGNC ID

HGNC:21699

ORF Size (aa)

1599

ORF Size (bp)

1599 bp

RefSeq ID

NM_201548

RefSeq Synonyms

NM_201548, NM_001160277, NM_001030313, NM_001030312, NM_001030311, BC137499, BC137498,

Species

human

UniGene ID

Hs.715753

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.