human CDK5R1 shRNA silencing AAV

Name
AAV-h-CDK5R1-shRNA

Cat No
shAAV-204848

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human CDK5R1.

shAAV-204848

AAV-h-CDK5R1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CDK5R1 (cyclin dependent kinase 5 regulatory subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a

Description (Vector)

CDK5R1 (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

Gene ID

8851

Gene Name (Vector)

cyclin dependent kinase 5 regulatory subunit 1

Gene Symbol

CDK5R1

HGNC ID

HGNC:1775

ORF Size (aa)

924

ORF Size (bp)

924 bp

RefSeq ID

NM_003885

RefSeq Synonyms

NM_003885, BC030792, BC026347, BC020580,

Species

human

UniGene ID

Hs.500015

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.