human CDC25B shRNA silencing AAV

Name
AAV-h-CDC25B-shRNA

Cat No
shAAV-204738

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human CDC25B.

shAAV-204738

AAV-h-CDC25B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CDC25B (cell division cycle 25B). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Gene ID

994

Gene Name (Vector)

cell division cycle 25B

Gene Symbol

CDC25B

HGNC ID

HGNC:1726

ORF Size (aa)

1743

ORF Size (bp)

1743 bp

RefSeq ID

NM_004358

RefSeq Synonyms

NM_021873, NM_021872, NM_004358, NM_001287524, NM_001287522, NM_001287520, NM_001287519, NM_001287518, NM_001287517, NM_001287516, BC051711, BC009953, BC006395,

Species

human

UniGene ID

Hs.153752

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.