human CD47 shRNA silencing AAV

Name 
AAV-h-CD47-shRNA
Cat No 
shAAV-204658
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human CD47.

shAAV-204658
AAV-h-CD47-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CD47 (CD47 molecule). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; CD antigen CD47; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen
Description (eCommerce)
CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. CD47 is a receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q08722].
Description (Vector)
CD47 encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
Gene ID
961
Gene Name (eCommerce)
CD47 molecule
Gene Name (Vector)
CD47 molecule
Gene Symbol
CD47
HGNC ID
HGNC:1682
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
918
ORF Size (bp)
918 bp
Protein Name (eCommerce)
Leukocyte surface antigen CD47
RefSeq ID
NM_001777
RefSeq Synonyms
NM_198793, NM_001777, BC037306, BC012884, BC010016,
Research Areas
Cardiovascular,Immunology,Neurobiology,Signal Transduction
Research Areas (Faceted)
cardiovascular,signal_transduction,immunology,neurobiology
Species
human
Target Sentence
CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. CD47 is a receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q08722].
UniGene ID
Hs.446414
UniProt ID (eCommerce)
Q08722

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.