human CCNT1 shRNA silencing AAV

Name
AAV-h-CCNT1-shRNA

Cat No
shAAV-204519

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human CCNT1.

shAAV-204519

AAV-h-CCNT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CCNT1 (cyclin T1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CCNT; CDK9-associated C-type protein; cyclin C-related protein; Cyclin-T; cyclin-T1; CYCT1; HIVE1; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1; MLLT10/CCNT1 fusion

Description (eCommerce)

Cyclin T1 is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, Cyclin T1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60563].

Description (Vector)

CCNT1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.

Gene ID

904

Gene Name (eCommerce)

cyclin T1

Gene Name (Vector)

cyclin T1

Gene Symbol

CCNT1

HGNC ID

HGNC:1599

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2181

ORF Size (bp)

2181 bp

Protein Name (eCommerce)

Cyclin-T1

RefSeq ID

NM_001240

RefSeq Synonyms

NM_001277842, NM_001240, BC172543, BC156506,

Research Areas

Cancer,Cell Cycle,Cell Division,Gene Expression,Host-Virus Interactions,RNA Binding,Signal Transduction,TGF-beta Signaling,TNF Signaling,Transcription Factor/Regulator,Transcriptional Misregulation in Cancer

Research Areas (Faceted)

cancer,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease,genetics,signal_transduction,immunology,genetics,transcription_translation,cancer

Species

human

Target Sentence

UniGene ID

Hs.279906

UniProt ID (eCommerce)

O60563

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.