human CBS shRNA silencing AAV

Name 
AAV-h-CBS-shRNA
Cat No 
shAAV-204180
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human CBS.

shAAV-204180
AAV-h-CBS-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CBS (cystathionine-beta-synthase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
beta-thionase; CBSL; cystathionine beta-synthase; Cystathionine beta-synthase-like protein; HIP4; methylcysteine synthase; serine sulfhydrase
Description (eCommerce)
Cystathionine beta-synthase (CBS) is the only known pyridoxal phosphate-dependent enzyme that contains heme. It is an important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury (By similarity).Cystathionine beta-synthase (CBS) acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. CBS is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor [taken from NCBI Entrez Gene (Gene ID: 875)].
Description (Vector)
CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. Multiple alternatively spliced transcript variants have been found for this gene.
Gene ID
875
Gene Name (eCommerce)
cystathionine-beta-synthase
Gene Name (Vector)
cystathionine-beta-synthase
Gene Symbol
CBS
HGNC ID
HGNC:1550
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1656
ORF Size (bp)
1656 bp
Protein Name (eCommerce)
Cystathionine beta-synthase
RefSeq ID
NM_000071
RefSeq Synonyms
NM_001321072, NM_001320298, NM_001178009, NM_001178008, NM_000071, BC011381, BC010242, BC007257, BC000440,
Research Areas
Cancer,Cardiovascular,Immunology,JNK Signaling,Metabolism/Metabolic Process,Neurobiology,Neurodevelopment,RNA Processing,Signal Transduction
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,genetics,immunology,metabolism,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Cystathionine beta-synthase (CBS) is the only known pyridoxal phosphate-dependent enzyme that contains heme. It is an important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury (By similarity).Cystathionine beta-synthase (CBS) acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. CBS is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor [taken from NCBI Entrez Gene (Gene ID: 875)].
UniGene ID
Hs.533013
UniProt ID (eCommerce)
P35520

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.