human CBFB shRNA silencing AAV

Name
AAV-h-CBFB-shRNA

Cat No
shAAV-204160

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human CBFB.

shAAV-204160

AAV-h-CBFB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CBFB (core-binding factor beta subunit). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CBF-beta; core-binding factor beta subunit; core-binding factor subunit beta; PEA2-beta; PEBP2B; PEBP2-beta; polyomavirus enhancer binding protein 2, beta subunit; Polyomavirus enhancer-binding protein 2 beta subunit; SL3/AKV core-binding factor beta subunit; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta

Description (eCommerce)

Core-binding factor subunit beta (CBF-beta) is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13951].

Description (Vector)

CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 (inv(16)(p13q22)) produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

Gene ID

865

Gene Name (eCommerce)

core-binding factor subunit beta

Gene Name (Vector)

core-binding factor beta subunit

Gene Symbol

CBFB

HGNC ID

HGNC:1539

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

549

ORF Size (bp)

549 bp

Protein Name (eCommerce)

Core-binding factor subunit beta

RefSeq ID

NM_001755

RefSeq Synonyms

NM_022845, NM_001755, BC018509,

Research Areas

Cancer,Immunology,Transcription Factor/Regulator

Research Areas (Faceted)

cancer,cell_biology,immunology,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.460988

UniProt ID (eCommerce)

Q13951

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.