human CAD shRNA silencing AAV

Name 
AAV-h-CAD-shRNA
Cat No 
shAAV-203935
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human CAD.

shAAV-203935
AAV-h-CAD-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Aspartate carbamoyltransferase; CAD protein; CAD trifunctional protein; CDG1; ZDihydroorotase; EIEE50; GATD4; multifunctional protein CAD
Description (eCommerce)
CAD is an enzyme involved in the first three steps of the de novo synthesis of pyrimidine nucleotides. CAD exhibits multiple catalytic activities and is described as a “fusion” protein that can function as a carbamoylphosphate synthetase, aspartate transcarbamoylase, and dihydroorotase. CAD has been shown to be phosphorylated by mitogen-activated protein (MAP) kinase and protein kinase A (PKA) for the coordination of cell cycle progression and pyrimidine biosynthesis.
Description (Vector)
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.
Gene ID
790
Gene Name (eCommerce)
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Gene Name (Vector)
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Gene Symbol
CAD
HGNC ID
HGNC:1424
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
6678
ORF Size (bp)
6678 bp
Protein Name (eCommerce)
CAD protein
RefSeq ID
NM_004341
RefSeq Synonyms
NM_004341, NM_001306079, BC065510,
Research Areas
Metabolism/Metabolic Process,Neurobiology
Research Areas (Faceted)
cell_biology,metabolism,neurobiology
Species
human
Target Sentence
CAD is an enzyme involved in the first three steps of the de novo synthesis of pyrimidine nucleotides. CAD exhibits multiple catalytic activities and is described as a “fusion” protein that can function as a carbamoylphosphate synthetase, aspartate transcarbamoylase, and dihydroorotase. CAD has been shown to be phosphorylated by mitogen-activated protein (MAP) kinase and protein kinase A (PKA) for the coordination of cell cycle progression and pyrimidine biosynthesis.
UniGene ID
Hs.377010
UniProt ID (eCommerce)
P27708

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.