human C12ORF5 shRNA silencing AAV

Name 
AAV-h-C12ORF5-shRNA
Cat No 
shAAV-202564
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TIGAR.

shAAV-202564
AAV-h-C12ORF5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TIGAR (TP53 induced glycolysis regulatory phosphatase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
C12orf5; FR2BP; fructose-2,6-bisphosphatase TIGAR; fructose-2,6-bisphosphate 2-phosphatase; probable fructose-2,6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator; TP53-induced glycolysis regulatory phosphatase; transactivated by NS3TP2 protein
Description (eCommerce)
TIGAR (TP53-induced glycolysis and apoptosis regulator) is a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis [taken from NCBI GeneID:57103].
Description (Vector)
C12orf5 is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.
Gene ID
57103
Gene Name (eCommerce)
TP53 induced glycolysis regulatory phosphatase
Gene Name (Vector)
TP53 induced glycolysis regulatory phosphatase
Gene Symbol
TIGAR
HGNC ID
HGNC:1185
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
813
ORF Size (bp)
813 bp
Protein Name (eCommerce)
Fructose-2,6-bisphosphatase TIGAR
RefSeq ID
NM_020375
RefSeq Synonyms
NM_020375, BC012340,
Research Areas
Apoptosis,Metabolism/Metabolic Process
Research Areas (Faceted)
cell_biology,metabolism
Species
human
Target Sentence
TIGAR (TP53-induced glycolysis and apoptosis regulator) is a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis [taken from NCBI GeneID:57103].
UniGene ID
Hs.504545
UniProt ID (eCommerce)
Q9NQ88

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.