human BOLA3 shRNA silencing AAV

Name
AAV-h-BOLA3-shRNA

Cat No
shAAV-202231

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human BOLA3.

shAAV-202231

AAV-h-BOLA3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human BOLA3 (bolA family member 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

MMDS2

Description (Vector)

BOLA3 encodes a protein that plays an essential role in the production of iron-sulfur (Fe-S) clusters for the normal maturation of lipoate-containing 2-oxoacid dehydrogenases, and for the assembly of the mitochondrial respiratory chain complexes. Mutation in this gene has been associated with multiple mitochondrial dysfunctions syndrome-2. Two alternatively spliced transcript variants encoding different isoforms with distinct subcellular localization have been reported for this gene (PMID:21944046).

Gene ID

388962

Gene Name (Vector)

bolA family member 3

Gene Symbol

BOLA3

HGNC ID

HGNC:24415

ORF Size (aa)

321

ORF Size (bp)

321 bp

RefSeq ID

NM_212552

RefSeq Synonyms

NM_212552, NM_001035505, BC153208, BC148671,

Species

human

UniGene ID

Hs.61472

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.