human BEST3 shRNA silencing AAV

Name
AAV-h-BEST3-shRNA

Cat No
shAAV-202100

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human BEST3.

shAAV-202100

AAV-h-BEST3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human BEST3 (bestrophin 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

VMD2L3

Description (Vector)

BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 (PubMed 12032738); Tsunenari et al., 2003 (PubMed 12907679)).(supplied by OMIM, Mar 2008)

Gene ID

144453

Gene Name (Vector)

bestrophin 3

Gene Symbol

BEST3

HGNC ID

HGNC:17105

ORF Size (aa)

2007

ORF Size (bp)

2007 bp

RefSeq ID

NM_152439

RefSeq Synonyms

NM_152439, NM_032735, NM_001282616, NM_001282615, NM_001282614, NM_001282613, BC166663, BC006440,

Species

human

UniGene ID

Hs.280782

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.