human B4GALT7 shRNA silencing AAV

Name
AAV-h-B4GALT7-shRNA

Cat No
shAAV-201920

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human B4GALT7.

shAAV-201920

AAV-h-B4GALT7-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human B4GALT7 (beta-1,4-galactosyltransferase 7). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

EDSP1; EDSSLA; XGALT1; XGPT1

Description (Vector)

B4GALT7 is a member of the beta-1,4-galactosyltransferase (beta4GalT) family. Family members encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose. Each beta4GalT member has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus which then remains uncleaved to function as a transmembrane anchor. The enzyme encoded by this gene attaches the first galactose in the common carbohydrate-protein linkage (GlcA-beta1,3-Gal-beta1,3-Gal-beta1,4-Xyl-beta1-O-Ser) found in proteoglycans. This enzyme differs from other beta4GalTs because it lacks the conserved Cys residues found in beta4GalT1-beta4GalT6 and it is located in cis-Golgi instead of trans-Golgi. Mutations in this gene have been associated with the progeroid form of Ehlers-Danlos syndrome.

Gene ID

11285

Gene Name (Vector)

beta-1,4-galactosyltransferase 7

Gene Symbol

B4GALT7

HGNC ID

HGNC:930

ORF Size (aa)

984

ORF Size (bp)

984 bp

RefSeq ID

NM_007255

RefSeq Synonyms

NM_007255, BC072403, BC062983, BC007317,

Species

human

UniGene ID

Hs.455109

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.