human APC shRNA silencing AAV

Name 
AAV-h-APC-shRNA
Cat No 
shAAV-201126
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human APC.

shAAV-201126
AAV-h-APC-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human APC (APC, WNT signaling pathway regulator). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
adenomatosis polyposis coli tumor suppressor; adenomatous polyposis coli (APC); adenomatous polyposis coli protein; BTPS2; deleted in polyposis 2.5; DP2; DP2.5; DP3; epididymis secretory sperm binding protein; GS; PPP1R46; Protein APC; protein phosphatase 1, regulatory subunit 46; truncated adenomatosis polyposis coli; WNT signaling pathway regulator
Description (eCommerce)
Adenomatosis polyposis coli (APC) is a tumor suppressor gene found to be mutated in most colon cancers and is the cause of familial adenomatous polyposis (FAP), an autosomal dominant disease that results in a predisposition to malignant colon cancer. APC has been shown to be involved in cell differentiation, migration, proliferation and adhesion. One function of APC involves its role as a scaffolding protein that regulates the phosphorylation and degradation of beta-catenin, a transcriptional regulator in the WNT signaling pathway. Additionally, APC has been found to be important in the regulation of microtubule arrays and mitotic spindle formation.
Description (Vector)
APC encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Gene ID
324
Gene Name (eCommerce)
APC, WNT signaling pathway regulator
Gene Name (Vector)
APC, WNT signaling pathway regulator
Gene Symbol
APC
HGNC ID
HGNC:583
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
8532
ORF Size (bp)
8532 bp
Protein Name (eCommerce)
Adenomatous polyposis coli protein
RefSeq ID
NM_000038
RefSeq Synonyms
NM_001127511, NM_001127510, NM_000038, BC113358, BC111930, BC111591, BC111462,
Research Areas
Angiogenesis,Apoptosis,Axonogenesis,BDNF Signaling,Cancer,Cell Cycle,Cell Division,Colorectal Cancer,Cytoskeleton,DNA Damage/Repair,HGF Signaling,Hippo Signaling,Host-Virus Interactions,Immunology,Kinase/Phosphatase,MAPK Signaling,MicroRNAs in Cancer,Neurobiology,Notch Signaling,Signal Transduction,Synapse,Tumor Suppressors/Oncoproteins,Wnt Signaling
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,developmental_biology,genetics,transcription_translation,immunology,infectious_disease,neurobiology,signal_transduction
Species
human
Target Sentence
Adenomatosis polyposis coli (APC) is a tumor suppressor gene found to be mutated in most colon cancers and is the cause of familial adenomatous polyposis (FAP), an autosomal dominant disease that results in a predisposition to malignant colon cancer. APC has been shown to be involved in cell differentiation, migration, proliferation and adhesion. One function of APC involves its role as a scaffolding protein that regulates the phosphorylation and degradation of beta-catenin, a transcriptional regulator in the WNT signaling pathway. Additionally, APC has been found to be important in the regulation of microtubule arrays and mitotic spindle formation.
UniGene ID
Hs.158932
UniProt ID (eCommerce)
P25054

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.