human ALAS1 shRNA silencing AAV

Product Info

Knockdown

>90% knockdown of mRNA in cultured cells

Viral Details

Viral Backbone

Recombinant AAV

AAV-ITR

AAV2

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

Promoter

U6

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Gene Details

Species

Human

Gene Symbol

ALAS1

Gene Name

5'-aminolevulinate synthase 1

Gene ID

211

Gene Synonyms

ALAS; ALAS-H; ALAS3; ALASH; MIG4

ORF Size

1923 bp

RefSeq#

NM_000688

Gene Description

ALAS1 encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniGene ID

Hs.476308

HGNC ID

HGNC:396