human AGL shRNA silencing AAV

Name 
AAV-h-AGL-shRNA
Cat No 
shAAV-200561
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human AGL.

shAAV-200561
AAV-h-AGL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human AGL (amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
GDE
Description (Vector)
AGL encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described.
Gene ID
178
Gene Name (Vector)
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Symbol
AGL
HGNC ID
HGNC:321
ORF Size (aa)
4599
ORF Size (bp)
4599 bp
RefSeq ID
NM_000028
RefSeq Synonyms
NM_000646, NM_000645, NM_000644, NM_000643, NM_000642, NM_000028, BC078663,
Species
human
UniGene ID
Hs.904

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.