human ACTG1 shRNA silencing AAV

Name
AAV-h-ACTG1-shRNA

Cat No
shAAV-200279

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ACTG1.

shAAV-200279

AAV-h-ACTG1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ACTG1 (actin gamma 1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ACT; ACTG; actin, cytoplasmic 2; cytoskeletal gamma-actin; DFNA20; DFNA26; epididymis luminal protein 176; Gamma-actin; HEL-176

Description (eCommerce)

Cytoskeletal beta-actin is one of six highly conserved actin isoforms that is expressed ubiquitously in eukaryotic cells. Cytoskeletal beta-actin functions to maintain cell structure and plays a role in motility.

Description (Vector)

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.

Gene ID

Gene Name (eCommerce)

actin gamma 1

Gene Name (Vector)

actin gamma 1

Gene Symbol

ACTG1

HGNC ID

HGNC:144

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1128

ORF Size (bp)

1128 bp

Protein Name (eCommerce)

Actin, cytoplasmic 2

RefSeq ID

NM_001614

RefSeq Synonyms

NM_001614, NM_001199954, BC053572, BC018774, BC015779, BC015695, BC015005, BC012050, BC010999, BC009848, BC007442, BC001920, BC000292,

Research Areas

Axon Guidance,Cancer,Cardiology,Cardiomyopathy,Cardiovascular,Chromatin Remodeling,Cytoskeleton,Hippo Signaling,Histone Modification,Host-Virus Interactions,Immunology,Innate Immunity,Neurobiology,Signal Transduction

Research Areas (Faceted)

cancer,cardiovascular,cell_biology,genetics,immunology,infectious_disease,neurobiology,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.514581

UniProt ID (eCommerce)

P63261

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.