human AAK1 shRNA silencing AAV

Name 
AAV-h-AAK1-shRNA
Cat No 
shAAV-200020
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human AAK1.

shAAV-200020
AAV-h-AAK1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human AAK1 (AP2 associated kinase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
adaptor-associated kinase 1; AP2-associated protein kinase 1
Description (eCommerce)
AAK1 (AP2-associated kinase 1) is a Ser/Thr protein kinase. The rat homolog was first identified in a screen of a rat brain cDNA library using a domain of the alpha subunit of the heterotetrameric AP2 complex as bait. The AP2 complex is one of two major coat proteins of endocytic vesicles that function in receptor-mediated endocytosis and the recycling of synaptic vesicles. AAK1 is responsible for phosphorylating the mu subunit of AP2 to promote clathrin-mediated endocytosis.
Description (Vector)
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Gene ID
22848
Gene Name (eCommerce)
AP2 associated kinase 1
Gene Name (Vector)
AP2 associated kinase 1
Gene Symbol
AAK1
HGNC ID
HGNC:19679
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2592
ORF Size (bp)
2592 bp
Protein Name (eCommerce)
AP2-associated protein kinase 1
RefSeq ID
NM_014911
RefSeq Synonyms
NM_014911, BC143710, BC111965, BC104842,
Research Areas
Kinase/Phosphatase,Notch Signaling,Signal Transduction,Transport
Research Areas (Faceted)
cell_biology,developmental_biology,signal_transduction
Species
human
Target Sentence
AAK1 (AP2-associated kinase 1) is a Ser/Thr protein kinase. The rat homolog was first identified in a screen of a rat brain cDNA library using a domain of the alpha subunit of the heterotetrameric AP2 complex as bait. The AP2 complex is one of two major coat proteins of endocytic vesicles that function in receptor-mediated endocytosis and the recycling of synaptic vesicles. AAK1 is responsible for phosphorylating the mu subunit of AP2 to promote clathrin-mediated endocytosis.
UniGene ID
Hs.468878
UniProt ID (eCommerce)
Q2M2I8

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.