mouse LIN28 Adenovirus
This is an Adenovirus expressing Mouse Rsg1.
ADV-280849
Ad-m-LIN28
Ready-to-use Adenovirus expressing Mouse Lin28a (lin-28 homolog A (C. elegans)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AL024421; ENSMUSG00000070700; Gm10299; Lin-28; lin-28A; Lin28; Tex17
Description (Vector)
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Gene ID
83557
Gene Name (Vector)
lin-28 homolog A (C. elegans)
Gene Symbol
Lin28a
ORF Size (aa)
630
ORF Size (bp)
630 bp
RefSeq ID
NM_145833
RefSeq Synonyms
NM_145833, BC068304,
Species
mouse
UniGene ID
Mm.302567
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.