mouse IRAK4 Adenovirus
This is an Adenovirus expressing Mouse Irak4.
ADV-280826
Ad-m-IRAK4
Ready-to-use Adenovirus expressing Mouse Irak4 (interleukin-1 receptor-associated kinase 4). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
8430405M07Rik; 9330209D03Rik; IRAK-4; NY-REN-64
Description (Vector)
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Gene ID
266632
Gene Name (Vector)
interleukin-1 receptor-associated kinase 4
Gene Symbol
Irak4
ORF Size (aa)
1380
ORF Size (bp)
1380 bp
RefSeq ID
NM_029926
RefSeq Synonyms
NM_029926, BC051676,
Species
mouse
UniGene ID
Mm.422858
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.