mouse WNK4 Adenovirus

Name 
Ad-m-WNK4
Cat No 
ADV-276301
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Mouse 1700040L02Rik.

ADV-276301
Ad-m-WNK4

Ready-to-use Adenovirus expressing Mouse Wnk4 (WNK lysine deficient protein kinase 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
2010002J11Rik; Pha2b; Prkwnk4
Description (Vector)
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L.
Gene ID
69847
Gene Name (Vector)
WNK lysine deficient protein kinase 4
Gene Symbol
Wnk4
ORF Size (aa)
3669
ORF Size (bp)
3669 bp
RefSeq ID
NM_175638
RefSeq Synonyms
NM_175638, BC096453,
Species
mouse
UniGene ID
Mm.23656

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.