human TFEB Adenovirus

Name 
Ad-h-TFEB
Cat No 
ADV-225358
Availability
4-5 weeks

This is an Adenovirus expressing Human TFEB.

ADV-225358
Ad-h-TFEB

Ready-to-use Adenovirus expressing Human TFEB (transcription factor EB). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ALPHATFEB; BHLHE35; class E basic helix-loop-helix protein 35; T-cell transcription factor EB; TCFEB; transcription factor EB
Description (eCommerce)
Transcription factor EB (TFEB) specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, TFEB activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression; thus, TFEB plays a central role in expression of lysosomal genes. TFEB also specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P19484].
Gene ID
7942
Gene Name (eCommerce)
transcription factor EB
Gene Name (Vector)
transcription factor EB
Gene Symbol
TFEB
HGNC ID
HGNC:11753
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1431
ORF Size (bp)
1431 bp
Protein Name (eCommerce)
Transcription factor EB
RefSeq ID
NM_007162
RefSeq Synonyms
NM_007162, NM_001271945, NM_001271944, NM_001271943, NM_001167827, BC032448,
Research Areas
Autophagy,Immunology,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,immunology,transcription_translation
Species
human
Target Sentence
Transcription factor EB (TFEB) specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, TFEB activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression; thus, TFEB plays a central role in expression of lysosomal genes. TFEB also specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P19484].
UniGene ID
Hs.715310
UniProt ID (eCommerce)
P19484

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.