human POLR2A Adenovirus

Name 
Ad-h-POLR2A
Cat No 
ADV-219386
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human POLR2A.

ADV-219386
Ad-h-POLR2A

Ready-to-use Adenovirus expressing Human POLR2A (RNA polymerase II subunit A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2
Description (eCommerce)
RNA polymerase II is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II subunit B1 (RPB1) is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit [taken from NCBI Entrez Gene (Gene ID: 5430)].
Description (Vector)
POLR2A encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Gene ID
5430
Gene Name (eCommerce)
RNA polymerase II subunit A
Gene Name (Vector)
RNA polymerase II subunit A
Gene Symbol
POLR2A
HGNC ID
HGNC:9187
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5913
ORF Size (bp)
5913 bp
Protein Name (eCommerce)
DNA-directed RNA polymerase II subunit RPB1
RefSeq ID
NM_000937
RefSeq Synonyms
NM_000937, BC137231, BC067295,
Research Areas
Chromatin Remodeling,DNA Damage/Repair,Gene Expression,Host-Virus Interactions,Huntington's Disease,Metabolism/Metabolic Process,mRNA Processing,mRNA Splicing,Neurobiology,Neurodegeneration,Phospho-specific,RNA Processing,Spliceosome,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,genetics,transcription_translation,infectious_disease,neurobiology,metabolism
Species
human
Target Sentence
RNA polymerase II is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II subunit B1 (RPB1) is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit [taken from NCBI Entrez Gene (Gene ID: 5430)].
UniGene ID
Hs.270017
UniProt ID (eCommerce)
P24928

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.