human LIN28B Adenovirus

Name 
Ad-h-LIN28B
Cat No 
ADV-213954
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human DRD1.

ADV-213954
Ad-h-LIN28B

Ready-to-use Adenovirus expressing Human LIN28B (lin-28 homolog B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CSDD2; Lin-28.2; lin-28B; protein lin-28 homolog B
Description (eCommerce)
Lin-28 homolog B (LIN28B) acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. LIN28B acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. LIN28B specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. It also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q6ZN17].
Description (Vector)
LIN28B belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation.
Gene ID
389421
Gene Name (eCommerce)
lin-28 homolog B
Gene Name (Vector)
lin-28 homolog B
Gene Symbol
LIN28B
HGNC ID
HGNC:32207
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
753
Protein Name (eCommerce)
Protein lin-28 homolog B
RefSeq ID
NM_001004317
RefSeq Synonyms
NM_001004317, BC137527, BC137526,
Research Areas
Cardiac Development,Cardiology,Cardiovascular,Mitochondrion,RNA Binding,RNA Processing,Zinc-finger
Research Areas (Faceted)
cardiovascular,cell_biology,developmental_biology,genetics,transcription_translation
Species
human
Target Sentence
Lin-28 homolog B (LIN28B) acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. LIN28B acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. LIN28B specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. It also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q6ZN17].
UniGene ID
Hs.23616
UniProt ID (eCommerce)
Q6ZN17

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.