human IDE Adenovirus

Name 
Ad-h-IDE
Cat No 
ADV-211766
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human IDE.

ADV-211766
Ad-h-IDE

Ready-to-use Adenovirus expressing Human IDE (insulin degrading enzyme). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Abeta-degrading protease; insulin protease; insulinase; insulin-degrading enzyme; INSULYSIN
Description (eCommerce)
Insulin-degrading enzyme (IDE) is a metalloendopeptidase that plays a primary role in the regulated degradation of insulin. In the cell, IDE is found mostly in the soluble cytosolic fraction, and small amounts exist in the plasma membrane, endosomes, and peroxisomes; however the highest relative concentration is found in the peroxisomes. IDE is proposed to be a multifunctional enzyme since it is also present in non-insulin sensitive cells. Other substrates of IDE include glucagon, IGF-II, atrial natriuretic factor, and TGF-alpha.
Description (Vector)
IDE encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.
Gene ID
3416
Gene Name (eCommerce)
insulin degrading enzyme
Gene Name (Vector)
insulin degrading enzyme
Gene Symbol
IDE
HGNC ID
HGNC:5381
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3060
ORF Size (bp)
3060 bp
Protein Name (eCommerce)
Insulin-degrading enzyme
RefSeq ID
NM_004969
RefSeq Synonyms
NM_004969, NM_001322797, NM_001322796, NM_001322795, NM_001322794, NM_001322793, NM_001165946, BC096339, BC096337, BC096336,
Research Areas
Alzheimer's Disease,Host-Virus Interactions,Insulin Signaling,Metabolism/Metabolic Process,Mitochondrion,Neurobiology,Neurodegeneration,Signal Transduction
Research Areas (Faceted)
cell_biology,infectious_disease,metabolism,neurobiology,signal_transduction
Species
human
Target Sentence
Insulin-degrading enzyme (IDE) is a metalloendopeptidase that plays a primary role in the regulated degradation of insulin. In the cell, IDE is found mostly in the soluble cytosolic fraction, and small amounts exist in the plasma membrane, endosomes, and peroxisomes; however the highest relative concentration is found in the peroxisomes. IDE is proposed to be a multifunctional enzyme since it is also present in non-insulin sensitive cells. Other substrates of IDE include glucagon, IGF-II, atrial natriuretic factor, and TGF-alpha.
UniGene ID
Hs.500546
UniProt ID (eCommerce)
P14735

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.