human GRLF1 Adenovirus

Name 
Ad-h-GRLF1
Cat No 
ADV-210544
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human CNTF.

ADV-210544
Ad-h-GRLF1

Ready-to-use Adenovirus expressing Human ARHGAP35 (Rho GTPase activating protein 35). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
glucocorticoid receptor DNA-binding factor 1; glucocorticoid receptor repression factor 1; GRF-1; GRLF1; P190A; P190-A; p190ARhoGAP; p190RhoGAP; rho GAP p190A; rho GTPase-activating protein 35
Description (eCommerce)
GRLF1 (glucocorticoid receptor DNA binding factor 1) was first identified as a factor that binds to the regulatory region of the human glucocorticoid receptor (GR) gene and represses GR transcription. Later, p190RhoGAP was identified as a protein that associates with p120RasGAP and through sequence comparison, p190RhoGAP and GRLF1 were determined to be one and the same. GRLF1/P190RhoGAP functions as a GTPase activating protein (GAP) for the Rho GTPase and plays an essential role in mediating cytoskeletal reorganization in response to growth factor signals. p190RhoGAP possesses an unusual GTP binding domain that may serve to regulate its GAP activity and the subsequent modulation of RhoGTPase activity. P190RhoGAP forms a significant complex with p120RasGAP. This association has been suggested to represent a method for the coordination of the Ras and Rho signaling pathways.
Description (Vector)
The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids.
Gene ID
2909
Gene Name (eCommerce)
Rho GTPase activating protein 35
Gene Name (Vector)
Rho GTPase activating protein 35
Gene Symbol
ARHGAP35
HGNC ID
HGNC:4591
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4500
ORF Size (bp)
4500 bp
Protein Name (eCommerce)
Rho GTPase-activating protein 35
RefSeq ID
NM_004491
RefSeq Synonyms
NM_004491, BC150257, BC003514,
Research Areas
Axon Guidance,Brain Development,Cancer,Cardiovascular,Cytoskeleton,EGFR Signaling,Immunology,Neurobiology,Neurodevelopment,PDGF Signaling,Rho Signaling,Signal Transduction,T-cell Receptor Signaling,Transcription Factor/Regulator,Tumor Suppressors/Oncoproteins
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,immunology,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
GRLF1 (glucocorticoid receptor DNA binding factor 1) was first identified as a factor that binds to the regulatory region of the human glucocorticoid receptor (GR) gene and represses GR transcription. Later, p190RhoGAP was identified as a protein that associates with p120RasGAP and through sequence comparison, p190RhoGAP and GRLF1 were determined to be one and the same. GRLF1/P190RhoGAP functions as a GTPase activating protein (GAP) for the Rho GTPase and plays an essential role in mediating cytoskeletal reorganization in response to growth factor signals. p190RhoGAP possesses an unusual GTP binding domain that may serve to regulate its GAP activity and the subsequent modulation of RhoGTPase activity. P190RhoGAP forms a significant complex with p120RasGAP. This association has been suggested to represent a method for the coordination of the Ras and Rho signaling pathways.
UniGene ID
Hs.509447
UniProt ID (eCommerce)
Q9NRY4

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.