human ATP6V1F Adenovirus

Name 
Ad-h-ATP6V1F
Cat No 
ADV-201768
Availability
4-5 weeks

This is an Adenovirus expressing Human ATP6V1F.

ADV-201768
Ad-h-ATP6V1F

Ready-to-use Adenovirus expressing Human ATP6V1F (ATPase H+ transporting V1 subunit F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ATP6S14; VATF; Vma7
Description (Vector)
ATP6V1F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.
Gene ID
9296
Gene Name (Vector)
ATPase H+ transporting V1 subunit F
Gene Symbol
ATP6V1F
HGNC ID
HGNC:16832
ORF Size (aa)
360
ORF Size (bp)
360 bp
RefSeq ID
NM_004231
RefSeq Synonyms
NM_004231, NM_001198909, BC107854, BC104231, BC104230,
Species
human
UniGene ID
Hs.78089

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.