mouse TWIST1 Over-expression AAV

Name 
AAV-m-TWIST1
Cat No 
AAV-275367
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Twist1.

AAV-275367
AAV-m-TWIST1

Ready-to-use AAV expressing Mouse Twist1 (twist basic helix-loop-helix transcription factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
bHLHa38; M-Twist; Pde; pdt; Ska10; Ska<m10Jus>; Twist
Description (Vector)
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
Gene ID
22160
Gene Name (Vector)
twist basic helix-loop-helix transcription factor 1
Gene Symbol
Twist1
ORF Size (aa)
621
ORF Size (bp)
621 bp
RefSeq ID
NM_011658
RefSeq Synonyms
NM_011658, BC083139, BC033434,
Species
mouse
UniGene ID
Mm.3280

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.