mouse TRIM28 Over-expression AAV
This is an AAV expressing Mouse Trim28.
AAV-274980
AAV-m-TRIM28
Ready-to-use AAV expressing Mouse Trim28 (tripartite motif-containing 28). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AA408787; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB-A-interacting protein; KRIP-1; MommeD9; RING-type E3 ubiquitin transferase TIF1-beta; Tif1b; Tif1beta; TIF1-beta; transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; tripartite motif protein 28; tripartite motif-containing protein 28
Description (eCommerce)
KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.
Description (Vector)
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6.
Gene ID
21849
Gene Name (eCommerce)
tripartite motif-containing 28
Gene Name (Vector)
tripartite motif-containing 28
Gene Symbol
Trim28
NCBI Taxonomy ID (eCommerce)
10090.0
ORF Size (aa)
2505
ORF Size (bp)
2505 bp
Protein Name (eCommerce)
Transcription intermediary factor 1-beta
RefSeq ID
NM_011588
RefSeq Synonyms
NM_011588, BC089337, BC058391,
Research Areas
Ubiquitination
Research Areas (Faceted)
cell_biology
Species
mouse
Target Sentence
KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.
UniGene ID
Mm.398345
UniProt ID (eCommerce)
Q62318
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.