mouse TRIM25 Over-expression AAV

Name 
AAV-m-TRIM25
Cat No 
AAV-274977
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Trim25.

AAV-274977
AAV-m-TRIM25

Ready-to-use AAV expressing Mouse Trim25 (tripartite motif-containing 25). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AA960166; AL022677; EFP; Zfp147
Description (Vector)
Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
Gene ID
217069
Gene Name (Vector)
tripartite motif-containing 25
Gene Symbol
Trim25
ORF Size (aa)
1881
ORF Size (bp)
1881 bp
RefSeq ID
NM_009546
RefSeq Synonyms
NM_009546, BC034276, BC006908,
Species
mouse
UniGene ID
Mm.248445

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.