mouse RFC1 Over-expression AAV

Name 
AAV-m-RFC1
Cat No 
AAV-270421
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Rfc1.

AAV-270421
AAV-m-RFC1

Ready-to-use AAV expressing Mouse Rfc1 (replication factor C (activator 1) 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
140kDa; Alp145; Recc1; RFC140
Description (Vector)
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA|Function: Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Gene ID
19687
Gene Name (Vector)
replication factor C (activator 1) 1
Gene Symbol
Rfc1
ORF Size (aa)
3399
ORF Size (bp)
3399 bp
RefSeq ID
NM_011258
RefSeq Synonyms
NM_011258, NM_001347358, NM_001347357, BC085173,
Species
mouse
UniGene ID
Mm.148877

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.