human TDG Over-expression AAV

Name 
AAV-h-TDG
Cat No 
AAV-225260
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human TDG.

AAV-225260
AAV-h-TDG

Ready-to-use AAV expressing Human TDG (thymine DNA glycosylase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
G/T mismatch-specific thymine DNA glycosylase; hTDG; Thymine-DNA glycosylase
Description (eCommerce)
Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine [taken from NCBI Entrez Gene (Gene ID: 6996)].
Description (Vector)
TDG belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
Gene ID
6996
Gene Name (eCommerce)
thymine DNA glycosylase
Gene Name (Vector)
thymine DNA glycosylase
Gene Symbol
TDG
HGNC ID
HGNC:11700
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1233
ORF Size (bp)
1233 bp
Protein Name (eCommerce)
G/T mismatch-specific thymine DNA glycosylase
RefSeq ID
NM_003211
RefSeq Synonyms
NM_003211, BC037557,
Research Areas
Chromatin Remodeling,DNA Damage/Repair,Epigenetic Regulation of Gene Expression,Immunology,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,immunology,genetics,transcription_translation
Species
human
Target Sentence
Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine [taken from NCBI Entrez Gene (Gene ID: 6996)].
UniGene ID
Hs.584809
UniProt ID (eCommerce)
Q13569

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.