human SF3B2 Over-expression AAV

Name 
AAV-h-SF3B2
Cat No 
AAV-222762
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human SF3B2.

AAV-222762
AAV-h-SF3B2

Ready-to-use AAV expressing Human SF3B2 (splicing factor 3b subunit 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Cus1; pre-mRNA splicing factor SF3b 145 kDa subunit; Pre-mRNA-splicing factor SF3b 145 kDa subunit; SAP 145; SAP145; SF3b1; SF3B145; SF3b150; spliceosome associated protein 145; Spliceosome-associated protein 145; splicing factor 3B subunit 2; splicing factor 3b, subunit 2, 145kD; splicing factor 3b, subunit 2, 145kDa
Description (eCommerce)
SF3b145/SAP145 is a 145 kDa subunit of the splicing factor 3B (SF3B) protein complex. The SF3B complex is composed of at least 8 subunits (SF3B1 through SF3B8). SF3A along with SF3B and a 12S RNA unit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. In this complex SF3B2 interacts directly with SF3B4 and associates with the pre-mRNA upstream of the branch site at the anchoring site. SF3A and SF3B may play a role in anchoring the U2 snRNP to the pre-mRNA upstream of the intron’s branch site.
Description (Vector)
SF3B2 encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
Gene ID
10992
Gene Name (eCommerce)
splicing factor 3b subunit 2
Gene Name (Vector)
splicing factor 3b subunit 2
Gene Symbol
SF3B2
HGNC ID
HGNC:10769
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1911
ORF Size (bp)
1911 bp
Protein Name (eCommerce)
Splicing factor 3B subunit 2
RefSeq ID
NM_006842
RefSeq Synonyms
NM_006842, BC007610,
Research Areas
Gene Expression,Host-Virus Interactions,mRNA Processing,mRNA Splicing,RNA Processing,Spliceosome
Research Areas (Faceted)
genetics,transcription_translation,infectious_disease,cell_biology
Species
human
Target Sentence
SF3b145/SAP145 is a 145 kDa subunit of the splicing factor 3B (SF3B) protein complex. The SF3B complex is composed of at least 8 subunits (SF3B1 through SF3B8). SF3A along with SF3B and a 12S RNA unit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. In this complex SF3B2 interacts directly with SF3B4 and associates with the pre-mRNA upstream of the branch site at the anchoring site. SF3A and SF3B may play a role in anchoring the U2 snRNP to the pre-mRNA upstream of the intron’s branch site.
UniGene ID
Hs.406423
UniProt ID (eCommerce)
Q13435

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.