human RPE65 Over-expression AAV

Name 
AAV-h-RPE65
Cat No 
AAV-221550
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human RPE65.

AAV-221550
AAV-h-RPE65

Ready-to-use AAV expressing Human RPE65 (RPE65, retinoid isomerohydrolase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
BCO3; LCA2; mRPE65; rd12; RP20; sRPE65
Description (Vector)
RPE65 encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.
Gene ID
6121
Gene Name (Vector)
RPE65, retinoid isomerohydrolase
Gene Symbol
RPE65
HGNC ID
HGNC:10294
ORF Size (aa)
1602
ORF Size (bp)
1602 bp
RefSeq ID
NM_000329
RefSeq Synonyms
NM_000329, BC075036, BC075035,
Species
human
UniGene ID
Hs.2133

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.