human POU5F1 Over-expression AAV

Name 
AAV-h-POU5F1
Cat No 
AAV-219487
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human POU5F1.

AAV-219487
AAV-h-POU5F1

Ready-to-use AAV expressing Human POU5F1 (POU class 5 homeobox 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Description (Vector)
POU5F1 encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Gene ID
5460
Gene Name (Vector)
POU class 5 homeobox 1
Gene Symbol
POU5F1
HGNC ID
HGNC:9221
ORF Size (aa)
1083
ORF Size (bp)
1083 bp
RefSeq ID
NM_002701
RefSeq Synonyms
NM_203289, NM_002701, NM_001285987, NM_001285986, NM_001173531, BC117437, BC117435,
Species
human
UniGene ID
Hs.249184

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.