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human POU5F1 shRNA silencing Adenovirus

Name 
Ad-h-POU5F1-shRNA
Cat No 
shADV-219487
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human POU5F1.

shADV-219487
Ad-h-POU5F1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human POU5F1 (POU class 5 homeobox 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
50
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Description (Vector)
POU5F1 encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Gene ID
5460
Gene Name (Vector)
POU class 5 homeobox 1
Gene Symbol
POU5F1
HGNC ID
HGNC:9221
ORF Size (aa)
1083
ORF Size (bp)
1083 bp
RefSeq ID
NM_002701
RefSeq Synonyms
NM_203289, NM_002701, NM_001285987, NM_001285986, NM_001173531, BC117437, BC117435,
Species
human
UniGene ID
Hs.249184

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.