human METAP2 Over-expression AAV

Name 
AAV-h-METAP2
Cat No 
AAV-215321
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human METAP2.

AAV-215321
AAV-h-METAP2

Ready-to-use AAV expressing Human METAP2 (methionyl aminopeptidase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MetAP 2; methionine aminopeptidase 2; MNPEP; p67; p67eIF2; Peptidase M; peptidase M 2; testicular tissue protein Li 17
Description (eCommerce)
Methionine aminopeptidase 2 (MetAP) removes the amino-terminal methionine from nascent proteins. The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P50579].
Description (Vector)
METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2.
Gene ID
10988
Gene Name (eCommerce)
methionyl aminopeptidase 2
Gene Name (Vector)
methionyl aminopeptidase 2
Gene Symbol
METAP2
HGNC ID
HGNC:16672
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1437
ORF Size (bp)
1437 bp
Protein Name (eCommerce)
Methionine aminopeptidase 2
RefSeq ID
NM_006838
RefSeq Synonyms
NM_006838, NM_001330246, NM_001317183, NM_001317182, BC013782,
Research Areas
Neurobiology,Signal Transduction
Research Areas (Faceted)
signal_transduction,neurobiology
Species
human
Target Sentence
Methionine aminopeptidase 2 (MetAP) removes the amino-terminal methionine from nascent proteins. The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P50579].
UniGene ID
Hs.444986
UniProt ID (eCommerce)
P50579

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.