human LIG4 Over-expression AAV

Name
AAV-h-LIG4

Cat No
AAV-213923

Availability
4-5 weeks

Categories
AAV, Over-Expression

This is an AAV expressing Human LIG4.

AAV-213923

AAV-h-LIG4

Ready-to-use AAV expressing Human LIG4 (DNA ligase 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CMV (default), or choose from 30 different ubiquitous or cell specific promoter

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

LIG4S

Description (Vector)

LIG4 is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Gene ID

3981

Gene Name (Vector)

DNA ligase 4

Gene Symbol

LIG4

HGNC ID

HGNC:6601

ORF Size (aa)

2736

ORF Size (bp)

2736 bp

RefSeq ID

NM_002312

RefSeq Synonyms

NM_206937, NM_002312, NM_001330595, NM_001098268, BC037491,

Species

human

UniGene ID

Hs.166091

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.