human GCLC Over-expression AAV

Name
AAV-h-GCLC

Cat No
AAV-209745

Availability
4-5 weeks

This is an AAV expressing Human GCLC.

AAV-209745

AAV-h-GCLC

Ready-to-use AAV expressing Human GCLC (glutamate-cysteine ligase catalytic subunit). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CMV (default), or choose from 30 different ubiquitous or cell specific promoter

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

GCL; GCS; GLCL; GLCLC

Description (Vector)

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Gene ID

2729

Gene Name (Vector)

glutamate-cysteine ligase catalytic subunit

Gene Symbol

GCLC

HGNC ID

HGNC:4311

ORF Size (aa)

1914

ORF Size (bp)

1914 bp

RefSeq ID

NM_001498

RefSeq Synonyms

NM_001498, NM_001197115, BC039894,

Species

human

UniGene ID

Hs.654465

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.