human ALAS1 Over-expression AAV

Name
AAV-h-ALAS1

Cat No
AAV-200713

Availability
4-5 weeks

This is an AAV expressing Human ALAS1.

AAV-200713

AAV-h-ALAS1

Ready-to-use AAV expressing Human ALAS1 (5′-aminolevulinate synthase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CMV (default), or choose from 30 different ubiquitous or cell specific promoter

Reporter

none, optional GFP, CFP, YFP, RFP or mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ALAS; ALAS-H; ALAS3; ALASH; MIG4

Description (Vector)

ALAS1 encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Gene ID

211

Gene Name (Vector)

5'-aminolevulinate synthase 1

Gene Symbol

ALAS1

HGNC ID

HGNC:396

ORF Size (aa)

1923

ORF Size (bp)

1923 bp

RefSeq ID

NM_000688

RefSeq Synonyms

NM_199166, NM_001304444, NM_001304443, NM_000688, BC011798,

Species

human

UniGene ID

Hs.476308

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.