eSYN promoter driven saCas9/CRISPR AAV AAV

Name 
AAV-eSYN-saCas9
Cat No 
7126
Availability
Immediate

Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex. Binding specificity is based on the gRNA and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.

The Cas9 from Streptococcus pyogenes SF370 (SpCas9) is too big to be packaged into AAV for most promoters, and there’s no extra room for including a sgRNA in the same payload with spCas9 in AAV. A small Cas9 from Staphylococcus aureus subsp. aureus (SaCas9) with high efficiency for cleaving mammalian endogenous DNA is the ideal solution to package into AAV along with sgRNA for effective gene modification in vivo.

This is an AAV-DJ (a synthetic serotype made from 8 wild type AAV including AAV2, 4, 5, 8, 9) expressing saCas9 nuclease under a neuronal specific eSYN promoter. The eSYN is a hybrid promoter consisting of a 0.4Kb CMV enhancer (e) and the 0.45Kb human Synapsin I promoter fragment (SYN). eSYN promoter provides 2-4 fold higher expression than that of the parental hSYN1 promoter. This AAV-eSYN-saCas9 can be package into other AAV serotypes upon request.

We also offer custom production service to package AAV-eSYN-saCas9 with your sgRNA in the same AAV virus. Please contact us for detail.

7126
AAV-eSYN-saCas9

Ready-to-use eSYN promoter driven saCas9/CRISPR AAV. 7126 AAV-eSYN-saCas9 saCas9 CRISPR

$495.00
Request Volume Pricing or Customization

Product Details

AAV Serotype
AAV-DJ
AAV ITR
AAV2
Promoter
eSYN ()
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
50

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.