human Synapsin 1 promoter driven saCas9/CRISPR AAV AAV

Name 
AAV-hSYN-saCas9
Cat No 
7124
Availability
Immediate

Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex. Binding specificity is based on the gRNA and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.

The main issue with Cas9 from Streptococcus pyogenes SF370 (SpCas9) is that this gene is too big to be packaged into AAV for most promoters, and there’s no extra room for a sgRNA with the spCas9 in same AAV. A small Cas9 from Staphylococcus aureus subsp. aureus (SaCas9) with high efficiency for cleaving mammalian endogenous DNA becomes the ideal solution to package into AAV for effective gene modification in vivo.

This is an AAV-DJ (a synthetic serotype made from 8 wild type AAV including AAV2, 4, 5, 8, 9) expressing spCas9 nuclease under a neuronal specific human synapsin 1 promoter. This AAV-hSYN-saCas9 can be package into other AAV serotypes upon request.

We also offer custom AAV production service to package AAV-hSYN-saCas9 with your sgRNA in the same AAV virus. Please contact us for detail.

7124
AAV-hSYN-saCas9

Ready-to-use human Synapsin 1 promoter driven saCas9/CRISPR AAV. 7124 Cas9 saCas9 AAV-hSYN-saCas9 CRISPR

$495.00
Request Volume Pricing or Customization

Product Details

AAV Serotype
AAV-DJ
AAV ITR
AAV2
Promoter
hSYN1 ()
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
50

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.