AAV-DJ-mCherry-iCre AAV

Name 
AAVDJ-mCherry-iCre
Cat No 
7095
Availability
Immediate
Categories
AAV, Cre Recombinases, Over-Expression

Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals. By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.

The AAV-DJ is a synthetic serotype made from DNA family shuffling of 8 wild type serotypes of AAV, including AAV2, 4, 5, 8, 9, avian, bovine and goat AAV. AAV-DJ outperformed standard AAV serotype 1-8 in cultured cells.

This AAV-DJ stock expresses an improved Cre (iCre) and a mCherry driven by one CMV promoter. The iCre and mCherry was separated by a 2A peptides.

7095
AAVDJ-mCherry-iCre

Ready-to-use AAV-DJ-mCherry-iCre. AAVDJ-mCherry-Cre AAV-DJ-mCherry-Cre AAVDJ-mCherry-iCre AAV-DJ-mCherry-iCre 7095

$495.00
Request Volume Pricing or Customization

Product Details

AAV Serotype
AAV-DJ
AAV ITR
AAV2
Promoter
CMV (ubiquitous)
Reporter
mCherry
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
20

Related Citations

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.