Category: AAV > Over-Expression > Cre Recombinases
Cat. No: 7093
Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals. By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.
The AAV-DJ is a synthetic serotype made from DNA family shuffling of 8 wild type serotypes of AAV, including AAV2, 4, 5, 8, 9, avian, bovine and goat AAV. AAV-DJ outperformed standard AAV serotype 1-8 in cultured cells.
This AAV-DJ stock expresses an improved Cre (iCre) and an eGFP driven by one CMV promoter. The iCre and eGFP was separated by a 2A peptides.
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- Viral Backbone
- Recombinant AAV
- AAV Serotype
- CMV (ubiquitous)
- Storage Buffer
- PBS/5% Glycerol
- 1x10^13 GC/ml
- Gene Symbol
- improved Cre (iCre)
This product is referenced in the following publications:
- D Zhang, etc (2016). Adiponectin regulates contextual fear extinction and intrinsic excitability of dentate gyrus granule neurons through AdipoR2 receptors. Molecular Psychiatry.
- F Sun, etc (2018). Adiponectin modulates ventral tegmental area dopamine neuron activity and anxiety-related behavior through AdipoR1. Molecular Psychiatry.
- CE Koch, etc (2020). Circadian regulation of hedonic appetite in mice by clocks in dopaminergic neurons of the VTA. Nature Communications.
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