AAV-mCherry-iCre (AAV1) AAV
Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.
By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.
This AAV1 stock expresses an improved Cre (iCre) along with mCherry under a control of the same CMV promoter. The Cre and mCherry was separated by a 2A peptides.
7086
AAV1-mCherry-iCre
Ready-to-use AAV-mCherry-iCre (AAV1). AAV1-mCherry-Cre AAV1-mCherry-iCre 7086
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.